Wireless Network for Behavioural Neuroscience (WNBN) https://pmc.ncbi.nlm.nih.gov/articles/PMC10180496/ This infrastructure enables remote, scalable, modular, and chronic high-throughput neuroscience studies. Using a custom-designed user interface and internet server for remote access, Bluetooth mesh technology for simultaneous and selective multi-control (i.e., a single remote for simultaneous and independently targeted control of spatially distributed aggregate of wireless devices), and affordable consumer hardware to enable mass deployment, the WNBN can be easily integrated into existing laboratory infrastructures at scale. Here we describe the operational concepts of the WNBN ecosystem for large scale in vivo control of freely behaving animals and provide proof-of-principle demonstrations of this ecosystem in established models of behavioural and circuit neuroscience. These approaches provide both a portable and built-in universal system that streamlines experimental workflows and enables previously untenable multiplexing of neural and behavioural manipulations. The WNBN enables perturbations in home cages and socially-interacting environments and this technology automates experimentation through a web-based schedule system. By measuring the locomotion, feeding, arousal and social behaviours of groups of mice or rats, we show that the system allows for bidirectional data transfer from readily available hardware, and that it can be used with programmable pharmacological or optogenetic stimulation. The WNBN system integrates wireless neural devices and other laboratory equipment with Bluetooth Low Energy (BLE) and Internet technologies to enable semi-automated and fully-automated behavioural studies, respectively. The wireless WNBN can either be controlled locally through BLE piconets (ad hoc network that connect the primary node and multiple secondary nodes using Bluetooth technology) using a smartphone or can be accessed globally over the Internet.